Resolving bundled microtubules using anti-tubulin nanobodies

نویسندگان

  • Marina Mikhaylova
  • Bas M C Cloin
  • Kieran Finan
  • Robert van den Berg
  • Jalmar Teeuw
  • Marta M Kijanka
  • Mikolaj Sokolowski
  • Eugene A Katrukha
  • Manuel Maidorn
  • Felipe Opazo
  • Sandrine Moutel
  • Marylin Vantard
  • Frank Perez
  • Paul M P van Bergen en Henegouwen
  • Casper C Hoogenraad
  • Helge Ewers
  • Lukas C Kapitein
چکیده

Microtubules are hollow biopolymers of 25-nm diameter and are key constituents of the cytoskeleton. In neurons, microtubules are organized differently between axons and dendrites, but their precise organization in different compartments is not completely understood. Super-resolution microscopy techniques can detect specific structures at an increased resolution, but the narrow spacing between neuronal microtubules poses challenges because most existing labelling strategies increase the effective microtubule diameter by 20-40 nm and will thereby blend neighbouring microtubules into one structure. Here we develop single-chain antibody fragments (nanobodies) against tubulin to achieve super-resolution imaging of microtubules with a decreased apparent diameter. To test the resolving power of these novel probes, we generate microtubule bundles with a known spacing of 50-70 nm and successfully resolve individual microtubules. Individual bundled microtubules can also be resolved in different mammalian cells, including hippocampal neurons, allowing novel insights into fundamental mechanisms of microtubule organization in cell- and neurobiology.

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2015